Work packages: WP2
Workpackages 2, 3 and 4 are all concerned with characterising the genetic resources available within Vigna umbellata in both Nepal and India, and collectively address project objective 2
- To assess genetic diversity and indigenous knowledge on ricebean in Nepal and India.
There has been no systematic attempt to collect Nepalese ricebean germplasm and no systematic collection of indigenous technical knowledge regarding the crop in either country? We will assess the genetic diversity of ricebean in Nepal and India, collect and catalogue germplasm, and simultaneously with this will collect and document indigenous knowledge on the crop. We will use both field assessment and molecular characterisation of the material.
Workpackage 2 addresses objective 2.1:
- To assess genetic diversity and uses of ricebean using indigenous knowledge of the crop
Ricebean germplasm will be collected to cover the widest possible range of diversity from each sampling site according to the names given to the landraces by farmers. Named landraces will be regarded as one “Farmer’s Unit of Diversity” (FUD) and samples of seed of one variety from one household will be regarded as one accession. The samples will be collected during baseline studies and surveys carried out to characterise several ecosites. An FUD could be the same as or different to another with the same name collected from another farmer: each will be given an accession number to allow identification.
Data on agromorphological traits of seeds, seedlings and established crops will be recorded at various growth stages, and will include phenological, qualitative and quantitative traits, including those used by farmers to distinguish landraces.
Diversity measures such as the Shannon Weaver Diversity Index (H’) for qualitative traits, coefficients of variation (CV) for quantitative traits, and multivariate statistics will be used to estimate the diversity of the material and relationships within it.
The endpoint of the workpackage will be an understanding of the distribution of ricebean in Nepal and India, and published analysis of its diversity and the reasons for it, as well as of the local knowledge of the crop.